Caspase-1 p20 抗体

Caspase-1 p20 Antibody is an unconjugated, approximately 20/46 kDa, rabbit-derived, anti-Caspase-1 p20 polyclonal antibody. Caspase-1 p20 Antibody can be used for: WB, ELISA, IHC-P, IHC-F, Flow-Cyt, ICC, IF expriments in human, mouse, rat, background without labeling.

12362

Predicted band size: 20/46kDa

affinity purified

Cytoplasm.

Non-conjugated

Unmodified

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat,

P29452

WB: 1:500-2000;ELISA: 1:5000-10000;IHC-P: 1:100-500;IHC-F: 1:100-500;Flow-Cyt: 1μg/Test;ICC: 1:100-500;IF: 1:100-500

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  Western blot analysis of extracts from M-spleen (lane 1) and 293T (lane 2) using Caspase-1 p20 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ELISA, IHC-P, IHC-F, FC, ICC/IF

液体

Supplied in 1% BSA, 0.01M PBS, 15mM Sodium Azide pH 7.4

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from mouse Caspase-1 p20: 181-280/402