CD8 抗体

CD8 Antibody is an unconjugated, approximately 27 kDa, rabbit-derived, anti-CD8 polyclonal antibody. CD8 Antibody can be used for: WB, IHC-P, IHC-F, IF expriments in human, mouse, background without labeling.

12525

Predicted band size: 27kDa

affinity purified

Membrane; Single-pass type I membrane protein.

Non-conjugated

Unmodified

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Mouse

Human

P01731

WB: 1:500-2000;IHC-P: 1:100-500;IHC-F: 1:100-500;IF: 1:100-500

• 
  Western blot analysis of extracts from M-Spleen (lane 1) using CD8 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-P, ICC/IF

液体

Supplied in 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from mouse CD8: 51-150/247