Cyclin B1 抗体

Cyclin B1 Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 48 kDa, targeting to Cyclin B1. It can be used for WB,IHC-F,IHC-P,ICC/IF,ELISA assays with tag free, in the background of Human, Mouse, Rat.

Cyclin B1：该基因编码的蛋白质是一种参与有丝分裂的调节蛋白。基因产物与 p34(cdc2) 复合形成成熟促进因子 (MPF)。编码的蛋白质对于正确控制细胞周期的 G2/M 过渡阶段是必需的。[由 RefSeq 提供，2017 年 8 月]

Free

891

Predicted band size: 48 kDa

affinity purified

Non-conjugated

Unmodified

Cell Biology

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

P14635

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200 ;ELISA: 1:10,000

• 
  Western blot analysis of extracts from Ramos (lane 1) and Hela (lane 2) and Raji (lane 3) and Jurkat (lane 4) using Cyclin B1antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-F, IHC-P, ICC/IF, ELISA

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Cyclin B1.AA range:91-140