DDX3 抗体 (YA784)

DDX3 Antibody (YA784) is a non-conjugated and Mouse origined monoclonal antibody about 73 kDa, targeting to DDX3 (6G8). It can be used for WB,ICC/IF,IP,ChIP assays with tag free, in the background of Human, Rat, Mouse, Monkey.

DDX3 (6G8)：由该基因编码的蛋白质是大型 DEAD-box 蛋白质家族的成员，该家族由保守的 Asp-Glu-Ala-Asp (DEAD) 基序的存在定义，并具有 ATP 依赖性 RNA 解旋酶活性。据报道，该蛋白质显示出高水平的不依赖于 RNA 的 ATPase 活性，并且与大多数 DEAD-box 解旋酶不同，ATPase 活性被认为受到 RNA 和 DNA 的刺激。该蛋白质具有多个保守结构域，被认为在细胞核和细胞质中均发挥作用。核作用包括转录调控、mRNP 组装、pre-mRNA 剪接和 mRNA 输出。在细胞质中，这种蛋白质被认为参与翻译、细胞信号传导和病毒复制。该基因的失调与肿瘤发生有关。该基因在 Y 染色体的非重组区有一个旁系同源物。在 4 号染色体和 X 染色体上发现了与该基因和 DDX3Y 旁系同源物具有相似性的假基因。可变剪接导致多个转录本变体。[由 RefSeq 提供，2014 年 10 月]

Free

1654

Predicted band size: 73 kDa

affinity purified

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Rat, Mouse, Monkey

O00571

WB: 1:500-1:1,000 ;IF: 1:50-1:200 ;IP: 1:20 ;ChIP: 1:20

• 
  Western blot analysis of extracts from Jurkat(lane 2(20μg) , K562(lane 3(20μg), Hela (lane 4(20μg) ) using DDX3 (HY-P80642) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80642, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8004, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF, IP, ChIP

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG2a

Endogenous

Synthetic peptide corresponding to Human DDX3.The exact sequence is proprietary to MCE.