eIF4B 抗体

eIF4B Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 69 kDa, targeting to eIF4B. It can be used for WB,IHC-P assays with tag free, in the background of Human.

eIF4B：启用 RNA 结合活性。预计参与真核翻译起始因子4F复合物的组装和翻译预起始复合物的形成。位于胞质溶胶中。自闭症谱系障碍和重度抑郁症的生物标志物。[由基因组资源联盟提供，2022 年 4 月]

Free

1975

Predicted band size: 69 kDa

affinity purified

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

P23588

WB: 1:500-1:1,000 ;IHC: 1:50-1:100

• 
  Western blot analysis of extracts from A549(lane 2(20μg) or lane 3(40μg)) and Hela(lane 4(20μg) or lane 5(40μg)), using eIF4B (HY-P80651) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80651, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human eIF4B.The exact sequence is proprietary to MCE.