Histone H4 抗体

Histone H4 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 11 kDa, targeting to Histone H4. It can be used for WB,ICC/IF,IHC-P assays with tag free, in the background of Human, Mouse, Rat.

Free

Predicted band size: 11 kDa

Protein A affinity purified.

Nucleus, Chromosome.

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human, Mouse, Rat

SwissProt: P62805 Human;SwissProt: P62806 Mouse;SwissProt: P62804 Rat

WB: 1:500-1:5,000 ;ICC/IF: 1:50-1:200 ;IHC-P: 1:50-1:200

• 
  Western blot analysis of extracts from Hela(lane 2(20μg), Jurkat(lane 3(20μg) and NIH3T3(lane 4(20μg) ) using Histone H4 (HY-P80178) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80178, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, ICC/IF, IHC-P

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Histone H4.AA range:54-103.