BNSP 抗体

BNSP Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 35 kDa, targeting to IBSP. It can be used for WB, IHC-P assays with tag free, in the background of Human, Mouse.

3381

Predicted band size: 35 kDa;Observed band size: 55 kDa

Non-conjugated

Unmodified

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Mouse, Rat

Human

P21815

WB: 1:500-2000;IHC-P: 1:100-500;IHC-F: 1:100-500;IF: 1:100-500;ELISA: 1:5000-10000;

• 
  Western blot analysis of extracts from M-Heart (lane 1) and C2C12 (lane 2) and HepG2 (lane 3) and 293T (lane 4) using BNSP antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/2000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-P, IHC-F, ICC/IF, ELISA

液体

Supplied in 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

KLH conjugated synthetic peptide derived from human Bone sialoprotein II: 21-120/317