Ku80 抗体 (YA714)

Ku80 Antibody (YA714) is a non-conjugated and Mouse origined monoclonal antibody about 83 kDa, targeting to Ku80 (8H1). It can be used for WB,ICC/IF,IP,ChIP assays with tag free, in the background of Human, Monkey.

Ku80 (8H1)：该基因编码的蛋白质是 Ku 异二聚体蛋白的 80 道尔顿亚基，也称为 ATP 依赖性 DNA 解旋酶 II 或 DNA 修复蛋白 XRCC5。Ku 是 DNA 依赖性蛋白激酶的 DNA 结合成分，它与 DNA 连接酶 IV-XRCC4 复合物一起通过非同源末端连接修复 DNA 双链断裂和完成 V(D) J重组事件。该基因在功能上补充了中国仓鼠 xrs-6，一种在 DNA 双链断裂修复和进行 V(D)J 重组方面有缺陷的突变体。该基因中罕见的微卫星多态性与不同放射敏感性患者的癌症相关。[由 RefSeq 提供，2008 年 7 月]

Free

7520

Predicted band size: 83 kDa

affinity purified

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Monkey

P13010

WB: 1:500-1:1,000 ;IF: 1:50-1:200 ;IP: 1:20 ;ChIP: 1:20

• 
  Western blot analysis of extracts from Hela (lane 1) and HepG2 (lane 2) and A549 (lane 3) using Ku80 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ICC/IF, IP, ChIP

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous

Synthetic peptide corresponding to Human Ku80.The exact sequence is proprietary to MCE.