MCU 抗体

MCU Antibody is a non-conjugated and Mouse origined monoclonal antibody about 40 kDa, targeting to MCU. It can be used for WB,FC assays with tag free, in the background of Human.

Free

Predicted band size: 40 kDa, Observed band size: 35 kDa

Protein G affinity purified.

Mitochondrion.

Non-conjugated

Unmodified

Cardiovascular

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human

SwissProt: Q8NE86 Human

WB: 1:500-1:1,000; FC: 1:50-1:100

• 
  Western blot analysis of extracts from HEK293(lane 2(20μg) or lane 3(40μg)) and HepG2(lane 4(20μg) or lane 5(40μg)), using MCU (HY-P80216) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80216, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, FC

液体

Supplied in 1*PBS (pH7.4), 0.2% BSA and 50% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgM

Endogenous

Synthetic peptide corresponding to Human MCU.AA range:51-325.