Phospho-MEK1 (Thr292) 抗体

Phospho-MEK1 (Thr292) Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 43 kDa, targeting to Phospho-MEK1 (Thr292). It can be used for WB assays with tag free, in the background of Mouse, Rat.

MEK1：由该基因编码的蛋白质是双重特异性蛋白激酶家族的成员，它充当丝裂原活化蛋白 (MAP) 激酶激酶。MAP 激酶，也称为细胞外信号调节激酶 (ERK)，充当多种生化信号的整合点。这种蛋白激酶位于 MAP 激酶的上游，并根据各种细胞外和细胞内信号刺激 MAP 激酶的酶活性。作为MAP激酶信号转导通路的重要组成部分，该激酶参与许多细胞过程，如增殖、分化、转录调控和发育。[由 RefSeq 提供，2008 年 7 月]

Free

5604

Predicted band size: 43 kDa

affinity purified

Non-conjugated

Phosphorylated

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Mouse, Rat

Q02750

WB: 1:500-1:1,000

• 
  Western blot analysis of extracts from C6(lane 2(20μg) or lane 3(40μg)), using Phospho-MEK1 (HY-P80833) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80833, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic phosphopeptide corresponding to residues surrounding Thr292 of Human MEK1.The exact sequence is proprietary to MCE.