2. 抗体
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  5. PKC epsilon 抗体

PKC epsilon 抗体

目录号: HY-P80434
COA 抗体使用指南

PKC epsilon Antibody 是一个非偶联、分子量约 84 kDa、兔源、抗 PKC epsilon 单克隆抗体。它可用于人、小鼠、大鼠背景下 WB、ICC、IHC-P、FC 实验，且不带标记。

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规格 价格 是否有货 数量
10 μL ￥470 In-stock
50 μL ￥1220 In-stock
100 μL ￥2000 In-stock
250 μL   询价  

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描述

PKC epsilon Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 84 kDa, targeting to PKC epsilon. It can be used for WB,ICC,IHC-P,FC assays with tag free, in the background of Human, Mouse, Rat.
标签

Free
分子量

Predicted band size: 84 kDa
纯度

Protein A affinity purified.
亚细胞定位

Cytoplasm.?Membrane.?Nucleus.
偶联

Non-conjugated
修饰

Unmodified
研究领域

Signal Transduction

产品类别

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
克隆性

Recombinant, Monoclonal
宿主

Rabbit
反应物种

Human, Mouse, Rat
蛋白数据库

SwissProt: Q02156 Human;SwissProt: P16054 Mouse;SwissProt: P09216 Rat
推荐稀释比例

WB: 1:500-1:1,000 ;ICC: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100

  • Western blot analysis of extracts from Jurkat (lane 1) and CaCO2 (lane 2) using PKC epsilon antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
应用

WB, ICC/IF, IHC-P, FC
性状

液体
组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
同型

IgG
敏感性

Endogenous
免疫原

Synthetic peptide corresponding to Human PKC epsilon.AA range:561-610.
文件资料

COA (193 KB)

抗体使用指南 (1083 KB)

PKC epsilon Antibody 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PKC epsilon Antibody
目录号：
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