PKR 抗体

PKR Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 62 kDa, targeting to PKR. It can be used for WB,ICC/IF,IHC-P,IP assays with tag free, in the background of Human.

Free

Predicted band size: 62 kDa

Protein A affinity purified.

Nucleus, Cytoplasm, perinuclear region.

Non-conjugated

Unmodified

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

SwissProt: P19525 Human

WB: 1:1,000-1:5,000 ;ICC/IF: 1:50-1:100 ;IHC-P: 1:50-1:100 ;IP: Use at an assay dependent concentration.

• 
  Western blot analysis of extracts from THP-1 (lane 1) and Jurkat (lane 2) and Hela (lane 3) using PKR antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, ICC/IF, IHC-P, IP

液体

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human PKR.AA range:500-540.