SIRT3 抗体

SIRT3 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 44 kDa, targeting to SIRT3. It can be used for WB,IHC-P assays with tag free, in the background of Human.

SIRT3：SIRT3 编码 III 类组蛋白脱乙酰酶的 sirtuin 家族成员，与酵母 Sir2 蛋白同源。编码的蛋白质只存在于线粒体中，它可以消除活性氧、抑制细胞凋亡并防止癌细胞的形成。SIRT3 对核基因表达、癌症、心血管疾病、神经保护、衰老和代谢控制具有深远影响。[由 RefSeq 提供，2019 年 5 月]

Free

23410

Predicted band size: 44 kDa

affinity purified

Non-conjugated

Unmodified

Epigenetics and Nuclear Signaling

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

Q9NTG7

WB: 1:500-1:1,000 ;IHC: 1:50-1:100

• 
  Western blot analysis of extracts from 293T (lane 1) and Hela (lane 2) using SIRT3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.

WB, IHC-P

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human SIRT3.The exact sequence is proprietary to MCE.