Thioredoxin 抗体

Thioredoxin Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 12 kDa, targeting to Thioredoxin. It can be used for WB,IHC-F,IHC-P,ICC/IF,IP assays with tag free, in the background of Human.

Thioredoxin：该基因编码的蛋白质作为同型二聚体，参与许多氧化还原反应。编码的蛋白质在某些蛋白质中半胱氨酸的可逆 S-亚硝基化中具有活性，这是对细胞内一氧化氮的反应的一部分。这种蛋白质存在于细胞质中。已发现该基因的两个转录本变体编码不同的亚型。[由 RefSeq 提供，2011 年 10 月]

Free

7295

Predicted band size: 12 kDa

affinity purified

Non-conjugated

Unmodified

Tags & Cell Markers

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

P10599

WB: 1:1000-1:10,000 ;IHC: 1:50-1:200 ;IF: 1:100-1:500 ;IP: 1:20

• 
  Western blot analysis of extracts from Jurkat(lane 2(20μg) and Jurkat(lane 3(40μg), Hela(lane 4(20μg) ) and Hela(lane 5(40μg) ) using Thioredoxin (HY-P80912) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80912, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-F, IHC-P, ICC/IF, IP

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human Thioredoxin/TRX.The exact sequence is proprietary to MCE.