2. Apoptosis
  3. TNF Receptor Apoptosis
  4. Atrosab

Atrosab 是一种人源化 IgG1 拮抗性抗 TNFR1 抗体。Atrosab 抑制 TNF 介导的细胞凋亡 (Apoptosis) 以及 IL-6 和 IL-8 的产生。Atrosab 可以减轻神经功能缺陷。Atrosab 可用于炎症性疾病的研究。建议同种型对照是 Human IgG1 kappa，Isotype Control (HY-P99001)。

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CAS No. : 1365659-05-7

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5 mg ￥7500
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Atrosab 相关抗体

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MCE 顾客使用本产品发表的 1 篇科研文献

查看 TNF Receptor 亚型特异性产品:

查看所有亚型
TNFRSF1A/CD120a TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Atrosab is a humanized IgG1 antagonistic anti-TNFR1 antibody. Atrosab inhibits TNF-mediated Apoptosis induction and IL-6 and IL-8 production. Atrosab reduces neurological deficits. Atrosab can be used for research of inflammatory disease. The recommend isotype control is Human IgG1 kappa, Isotype Control (HY-P99001)[1][2][3].

同型

Human IgG1 kappa
推荐同型对照抗体
种属

Humanized

体外研究
(In Vitro)

Atrosab (0.1-1000 nM, 先用抗体预处理1 h，然后加刺激剂 TNF 共同孵育 7 h) 以剂量依赖的方式抑制 TNF 诱导的 Kym-1 细胞凋亡[1]。
Atrosab (0.1-1000 nM, 先用抗体预处理1 h，然后加刺激剂 TNF 共同孵育 7 h) 以剂量依赖的方式抑制 TNF 诱导的 HeLa 细胞释放 IL-6 和 HT1080 细胞释放 IL-8[1]。
Atrosab (100 μg/mL,24 h) 减少内皮细胞黏附分子的表达和 T 细胞的黏附[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Atrosab 相关抗体:

Cell Viability Assay[1]

Cell Line: Kym-1 cells
Concentration: 0.1-1 μM
Incubation Time: preincubate with antibody for 1 h, then add the stimulant TNF and co-incubate for 7 hours
Result: Exhibited half-maximal cytotoxicity at 60 nM in Kym-1 cells.
Inhibited IL-6 release from HeLa cells with an IC50 of 60 nM.
Inhibited IL-8 release from HT1080 cells with an IC50 of 60 nM.
体内研究
(In Vivo)

Atrosab (i.v., 一周两次, 28 天) 在猴子和人 TNF 转基因/人 TNFR1 敲除小鼠模型和人 TNFR1 敲除小鼠 (hp55K/i) EAE 模型模型中都显示出对炎症性疾病，如类风湿性关节炎 (RA) 和多发性硬化症 (MS) 有效[2]。
Atrosab (20 mg/kg, i.p.) 在人源化 TNFR1 基因敲入小鼠中免疫细胞浸润显著减少[3]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: CIA rhesus monkey model[2].
Dosage:
Administration: i.v., twice weekly, 28 days
Result: Reduced of the acute-phase C-reactive protein (CRP) and IL-6 in serum.
Improved the clinical arthritis score, delayed the onset of symptoms, and inhibited cartilage degradation.
Animal Model: Human TNF transgenic/human TNFR1 Knock-in mice model (Tg197/hp55K/i) for arthritis and human TNFR1 Knock in mice (hp55K/i) EAE model for multiple sclerosis(MS)[2].
Dosage:
Administration: i.v., twice weekly, 28 days
Result: Significantly reduced arthritic pathology and the underlying histopathological lesions in rheumatoid arthritis (RA) mice.
Abolished the development of paralytic symptoms without affecting the overall health status of the animals in EAE mice model of MS.
Showed to be effective for treatment of inflammatory diseases such as RA and MS.
Animal Model: humanized TNFR1 knock-in mice[3].
Dosage: 20 mg/kg
Administration: i.p., four times (on days 1, 4, 8 and 12 of manifest EAE)
Result: Significantly prevented EAE-associated weight loss.
Led less spinal cord demyelination.
Reduced accumulated amyloid precursor protein (APP), a marker of axonal injury.
Reduced numbers of CD3+ T cells, Mac-3+ activated microglia/macrophages and CD45R+ B cells.
Reduced immune cell infiltration.
CAS 号
性状

液体

颜色

Colorless to light yellow

运输条件

Shipping with dry ice.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Format
  • Human IgG1 kappa
Biological Activity
  • Immobilized Human TNFR1,His Tag can bind Atrosab. The EC50 for this effect is 8.161 ng/mL.
  • Loaded Atrosab on AHC2 biosensor, can bind TNFRSF1A Protein, Human (His, HY-P70801) with an affinity constant of 1.151E-08 M as determined in BLI assay.
纯度 & 产品资料
参考文献

Atrosab 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Atrosab1365659-05-7TNF ReceptorApoptosisTumor Necrosis Factor ReceptorTNFRinflammationantibodyCHO cellsneuroprotectionInhibitorinhibitorinhibit

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目录号：
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