1. Biology Dictionary
  2. Bimolecular Luminescence Complementation

Bimolecular Luminescence Complementation双分子发光互补

释义:

The bimolecular luminescence complementation (BiLC) is an assay for determination of protein interactions and/or their location in living cells. This approach is based on complementation between two fragments of a bioluminescent enzyme such as luciferin or aequorin. Interactions between proteins fused to each fragment bring the fragments together resulting in the reconstitution of a fully functional bioluminescent enzyme that can be identified through microscopy or luminescence detection.

双分子生物发光互补 (BiLC) 是一种用于确定蛋白质相互作用和/或其在活细胞中定位的测定法。该方法基于双分子荧光素酶 (如荧光素或水母荧光素) 的两个片段之间的互补。融合到每个片段的蛋白质之间的相互作用使片段靠得足够近,从而重新组装并成为功能性的荧光素酶,可以通过显微镜或发光检测进行鉴定。

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