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  2. Gal4 VP16 Complementation

Gal4 VP16 ComplementationGAL4-VP16 互补法

释义:

A chimeric protein consisting of the GAL4 DNA-binding domain (aa 1-147 of GAL4) and a transcriptional activation domain from the herpes simplex virus protein VP16 (either aa 411-490 or aa 411-455) can specifically activate transcription of a reporter gene located downstream ofGAL4 DNA binding sites and the E1B minimal promoter. Similarly, two chimeric proteins, one encoding a chimeric GAL4 protein and the other encoding a chimeric VP16 protein, can activate the reporter gene, if the domains fused to the GAL4 and VP16 sequences can complex with appropriate conformation. However, if the domains fused to the GAL4 and VP16 sequences do not interact specifically to form a + complex that reconstitutes GAL4 function, the reporter gene cannot be activated.

一种由 GAL4 DNA 结合域 (GAL4 的 1-147 氨基酸) 和来自单纯疱疹病毒蛋白 VP16 的转录激活域 (可选 411-490 或 411-455 氨基酸) 构成的嵌合蛋白,可以特异性激活位于 GAL4 DNA 结合位点下游的报告基因的转录。类似地,两个嵌合蛋白,一个编码嵌合 GAL4 蛋白,另一个编码嵌合 VP16 蛋白,也可以激活报告基因,只要融合到 GAL4 和 VP16 序列的结构域能以适当的构象形成复合物。然而,如果融合到 GAL4 和 VP16 序列的结构域未能特异性地相互作用形成复合物,报告基因则无法被激活。

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