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  2. In-Gel Kinase Assay

In-Gel Kinase Assay凝胶内激酶活性测定实验

释义:

In-Gel Kinase Assay is a technique where kinases transfer radioactive phosphate isotopes from nucleotides to substrate proteins for labeling. The labeled substrates are then separated by gel electrophoresis, and the presence of the phosphate label is confirmed via autoradiography. This method is commonly used to study kinase activity, substrate specificity, and their roles in signaling pathways.

凝胶内激酶活性测定实验通过激酶将磷酸同位素从核苷酸转移到底物蛋白上实现底物的放射性标记。标记的底物随后通过凝胶电泳进行分离,并通过放射自显影确认磷酸标记的存在。此技术常用于研究激酶的活性、底物特异性及其在信号通路中的作用。

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