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  2. LexA VP16 Transcription Complementation

LexA VP16 Transcription ComplementationLexA VP16 转录互补法

释义:

LexA VP16 Transcription Complementation uses the yeast two-hybrid system, combining the Escherichia coli LexA amino acids 1-202 as the DNA-binding domain and the transcriptional activation domain from the herpes simplex virus protein VP16 to specifically activate the transcription of a downstream reporter gene. This method is particularly important in protein-protein interaction and gene regulation research.

LexA VP16 转录互补法使用酵母双杂交系统,结合大肠杆菌 LexA氨基酸 1-202 作为 DNA 结合域和来自单纯疱疹病毒蛋白 VP16 的转录激活域,特异性地激活下游报告基因的转录。此方法在蛋白质相互作用和基因调控研究中尤为重要。

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