1. Biology Dictionary
  2. RNA Immunoprecipitation

RNA ImmunoprecipitationRNA 免疫共沉淀法

释义:

RNA Immunoprecipitation is used to study the specific interaction mechanisms between RNA and proteins. The method chemically cross-links proteins to RNA (e.g., using formaldehyde), and then immunoprecipitates the target protein to separate the cross-linked complex. RNA is released by reversing the cross-link, followed by RNA analysis using reverse transcription PCR or RNA-Seq, which quantifies RNA types and abundance bound to proteins.

RNA 免疫共沉淀法可用于研究 RNA 和蛋白质之间的特异性相互作用机制。该方法通过化学交联 (如使用甲醛) 将蛋白质与 RNA 固定,然后利用特异性抗体对目标蛋白进行免疫沉淀分离交联复合物。接着,通过逆转交联释放 RNA,结合逆转录 PCR 或 RNA-Seq 进行 RNA 分析,可定量研究蛋白质结合的 RNA 种类和丰度。

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