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  2. Viral Particle Co-Purification

Viral Particle Co-Purification病毒颗粒共纯化

释义:

Viral Particle Co-Purification is used to capture interaction partners (prey) in viral-like particles (VLPs), combined with techniques such as mass spectrometry (MS) and Western blotting to identify multimeric complexes and binary interactions. This method captures the prey into VLPs by fusing it with viral proteins (e.g., HIV-1 GAG protein).

病毒颗粒共纯化用于捕获病毒样颗粒 (VLPs) 中的相互作用伙伴 (猎物), 结合质谱 (MS) 和蛋白质印迹等技术,识别多聚体复合物及二元相互作用。该方法通过诱饵与病毒蛋白 (如 HIV-1 GAG 蛋白) 的融合,将猎物捕获到 VLPs 中。

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