2. Biology Dictionary
  3. Zymography Methods

Zymography Methods酶谱分析法

释义：

Zymography is used to measure the activity of enzymes and is widely applied in enzyme research, metabolic pathway analysis, and drug screening. Samples are run in a gel containing gelatin under non-reducing conditions. The gel is then incubated under enzyme activity conditions, and after staining with Coomassie Brilliant Blue, areas of gelatin depletion are used as an indicator of enzyme activity. This method provides an intuitive reflection of the enzyme's action on its substrate.

酶谱分析法用于测定酶的活性，广泛应用于酶学研究、代谢途径解析和药物筛选等领域。样品在含有明胶的凝胶中运行，且在非还原条件下进行。随后将凝胶置于酶活性条件下孵育，用考马斯亮蓝染色后，明胶缺失的区域被视为酶活性的测量指标，通过这种方法可以直观地反映酶对底物的作用情况。

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