Bovin beta-1,4-galactosyltransferase 1 (Y289L)  (Synonyms: Bovin B4GALT1 (Y289L))

Bovin beta-1,4-galactosyltransferase 1 (Y289L) (Bovin B4GALT1 (Y289L)) is a mutated form of bovine-derived galactosyltransferase with a mutation at the Y289L genetic site. Bovin beta-1,4-galactosyltransferase 1 can label O-GlcNAcylated proteins with an N-azidoacetylgalactosamine (GalNAz) group. This labeling method allows for the specific, unbiased, and global labeling of O-GlcNAcylated proteins. After labeling, the appended azide group can react with a wide variety of alkyne-modified chemical probes, facilitating multiple downstream analyses^[1].

产品信息
Bovin B4GALT1(Y289L) 是一种突变型半乳糖转移酶，能有效转移 GalNAc 或者 GalNAz到末端 GlcNAc 残基，后者可以应用于抗体-药物偶联物 (ADC) 作为靶向癌症治疗等。本品为重组表达，表达系统 HEK293 cell，C-terminal His Tagged，分子量 50.8 kDa，所使用的保存缓冲液为 20 mM Tris-HCl (pH 7.4), 200 mM NaCl，20% Glycerin。

使用说明
反应体系：
50 mM Tris-HCl (PH 7.5)，6 mM MnCl2;
反应试剂：
UDP-GalNAz：抗体（当量比）= 300:1；
酶添加量：酶/抗体=5%；
按照上述条件配制反应液，37℃ 反应 48 小时，后置于 - 15~- 25℃ 停止反应并进行样品检测。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

液体

Colorless to light yellow

牛 β-1,4-半乳糖基转移酶 1 (Y289L)

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Thompson JW, et al. Methods for the Detection, Study, and Dynamic Profiling of O-GlcNAc Glycosylation. Methods Enzymol. 2018;598:101-135.  [Content Brief]