Carboxypeptidase B (MS grade)  (Synonyms: 羧肽酶 B（MS 级）; EC 3.4.2.2 (MS grade))

Carboxypeptidase B (MS grade) is a peptide exonuclease that can specifically degrade peptide chains. Carboxypeptidase B (MS grade) is progressively degraded from the C-terminal to release free amino acids. Carboxypeptidase B (MS grade) hydrolyzes only peptide bonds with basic amino acids (such as arginine and lysine) as C-terminal residues^[1][2].

本产品可用于： 1）抗体碱性峰的检测; 2）蛋白质 C-末端氨基酸的测定 。
使用方法
1）溶解：将羧肽酶 B 以终浓度 1-10 mg/ml 溶解在纯水或 25 mM Tris-HCl（pH 7.5-8.0）中。
2）酶切：羧肽酶 B 和目的蛋白推荐比例为 1:20-1:100（w:w）进行酶切，最适酶切 pH 为 7.0-9.0。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

9025-24-5

固体

White to off-white

羧肽酶 B（MS 级）

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. FOLK JE, et al. The kinetics of carboxypeptidase B activity. II. Kinetic parameters of the cobalt and cadmium enzymes. J Biol Chem. 1962 Oct;237:3100-4.  [Content Brief]

  [2]. Qian X, et al. Molecular characterization of carboxypeptidase B-like (CPB) in Scylla paramamosain and its role in white spot syndrome virus and Vibrio alginolyticus infection. Fish Shellfish Immunol. 2019 Nov;94:434-446.  [Content Brief]