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  3. Fluorescent Dye
  4. Cypate

Cypate 是一种花青染料，是一种用于体内肿瘤成像的近红外 (NIR) 荧光探针。

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Cypate Chemical Structure

Cypate Chemical Structure

CAS No. : 95837-47-1

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Cypate 的其他形式现货产品:

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Cypate 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Cypate, a cyanine dye, is a near infrared (NIR) fluorescent probe for in vivo tumor imaging[1][2].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. 将每种类型细胞在单独的 35 毫米玻璃底培养皿上接种 1×106 个。
2. 24 小时后，将细胞与 10 µM Cypate 无酚红培养基溶液一起孵育 2 小时。
3. 处理后，细胞用 PBS 洗涤 3 次并用 4% 多聚甲醛固定用于成像，或收集细胞内的 Cypate 内容物用于光谱和/或 LC-MS 分析。
4. 对体外细胞培养实验进行共聚焦显微镜检查。Cypate 的激发波长为 647 nm[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cypate 相关抗体:
体内研究
(In Vivo)

Cypate (10 nmol; 100 µL; 静脉给药; 每 24 小时一次; 连续 6 天) 导致肝脏蓄积最高[1]。
Cypate (5 mg/kg; 静脉给药) 在具有 4T1 细胞的 Balb/c 小鼠肿瘤中的荧光信号微弱并迅速衰减，这可能是由于游离 Cypate 从体内快速消除所致。在游离cypate组中观察到肝脏和肾脏中更强的荧光。使用 IVIS Lumina 成像系统 (Ex-745 nm; Em-800 nm) 评估 Cypate 荧光信号[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Foxn1nu/Foxn1nu nude 2.5-month-old female mice with breast cancer cells (MDA-MB-231 Luc2)[1]
Dosage: 10 nmol in 100 µL PBS
Administration: IV; every 24 hours for 6 days
Result: The accumulation alone in the tumor was negligible for 24 hours after the injection.
The liver had the highest accumulation at all time points.
分子量

624.77

Formula

C41H40N2O4
CAS 号
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

Cypate 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cypate95837-47-1Fluorescent DyeCyaninedyenear infraredNIRvivo tumor imagingA549Inhibitorinhibitorinhibit

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目录号：
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