DDAO-C6 

DDAO-C6 is a cridone ester derivative, highly specific fluorescence for detecting human serum albumin (HSA). DDAO-C6 acts as an enzymatic activatable near-infrared fluorescent probe in visually sensing endogenous lipase from gut microbes (Ex/Em=600/658 nm)^[1][2].

DDAO-C6 (10 μM; 30 min; 37 ℃) shows a linear relationship between the fluorescence intensities and lipase concentrations (0-50 μg/mL), and products a red fluorescence signal in the presence of active lipase^[1].
DDAO-C6 hydrolysates have fluorescence properties, with excitation wavelength=550-600 nm, emission spectrum=630-700 nm^[2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Intestinal Fungi^[1]:
1. Disperse fresh intestinal secretions in sterile water and coate on a potato agar plate, containing penicillin (100 U/mL)/streptomycin (0.1 mg/mL) to inhibit intestinal bacteria. 2. Culture at 32 °C for about 5 days until the development of obvious fungal colonies. 3. Spray DDAO-C6 (100 μM) on colonies and incubate at 32 °C for 2 h. 4. Image plate samples on a Amersham Typhoon RGB (λex = 635 nm, λem = 670 ± 15 nm).

DDAO-C6 can be used to guide the rapid identification and cultivation of lipase active fungal strains from intestinal microbes in human feces, which is a potential technique for the biological investigation of intestinal microbes^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

420.33

C₂₂H₂₃Cl₂NO₃

2102418-90-4

658

600

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Feng L, et al. Visual Identification of Trichosporon asahii, a Gut Yeast Associated with Obesity, Using an Enzymatic NIR Fluorescent Probe. Anal Chem. 2022.  [Content Brief]

  [2]. Cui J, et al. Application of highly specific fluorescent probe for detection of human serum albumin: China, CN106841128[P]. 2017-06-13.