Dextranase, Trichoderma reesei  (Synonyms: 葡聚糖酶)

Dextranase, Trichoderma reesei, glucan hydrolase, is often used in biochemical research. Dextranase can catalyze the hydrolysis of α-(1,6)-glucosidic bonds in dextran, and has a wide range of applications, such as food processing, sugar modification, agent preparation, and medicine for enhancing the activity of endocarditis antibiotics wait^[1].

产品信息
分子量: 64.6 kDa (Theoretical)
最适 Ph 范围: 5.0-7.0
激活剂: Co²⁺, Mn²⁺, Cu²⁺
抑制剂: Hg²⁺, Ag²⁺, N-bromosuccinimide, I ₂

使用说明
1. 溶解：可溶于纯水（1 mg/mL），建议新鲜配制。或根据具体实验参考文献 buffer 溶解。
2. 稀释：使用前立即进一步稀释至 5-20 μg/mL。
3. 酶解：1.9 mL 2% 葡聚糖底物在 37°C 水浴中孵育，向其中加入 0.1 mL 的酶稀释液。在 37°C 下孵育 30 分钟。通过定时将 1 mL 酶底物混合物移至含有 1 mL 二硝基水杨酸试剂的试管中来停止反应。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

9025-70-1

固体

Light yellow to light brown

葡聚糖酶

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Nayera A M Abdelwahed, et al. Application of statistical design for the optimization of dextranase production by a novel fungus isolated from Red Sea sponge. 3 Biotech. 2014 Oct;4(5):533-544.   [Content Brief]