Dnp-PLGLWA-DArg-NH2 TFA 

Dnp-PLGLWA-DArg-NH2 TFA is a fluorogenic substrate for MMP-1 and MMP-9. Dnp-PLGLWA-DArg-NH2 TFA can be used to quantify the activity of MMPs (Ex=280 nm, Em=360 nm)^[1][2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[2].
1. Enzyme activity assays are performed in 50 mmol/l Tris-HCl buffer, pH 7.5, 0.15 mol/l NaCl, 10 mmol/l CaCl₂, 0.02% TNC buffer containing 0.05% Brij 35 and 50 μM ZnSO₄.
2. Dnp-PLGLWA-DArg-NH2 TFA. Each fraction is incubated with 1 μM substrate at 37°C for 20 h.
3. Stop the reaction by the addition of 3% acetic acid.
4. Measure the fluorescence using wavelengths of 280 nm (excitation) and 360 nm (emission) with a fluorescence reader.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1091.10

C₄₇H₆₅F₃N₁₄O₁₃

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. G M McGeehan, et al. Characterization of the peptide substrate specificities of interstitial collagenase and 92-kDa gelatinase. Implications for substrate optimization. J Biol Chem. 1994 Dec 30;269(52):32814-20.  [Content Brief]

  [2]. Ken-ichi Shimokawa Ki, et al. Matrix metalloproteinase (MMP)-2 and MMP-9 activities in human seminal plasma. Mol Hum Reprod. 2002 Jan;8(1):32-6.  [Content Brief]