1. 糖生物学酶
  2. Endo-β-N-acetylglucosaminidase F2

Endo-β-N-acetylglucosaminidase F2  (Synonyms: Endo F2)

目录号: HY-E70133
COA 产品使用指南

Endo-β-N-acetylglucosaminidase F2 (Endo F2) 是一种高度特异性的糖苷内切酶,可在天冬酰胺连接的复合双触角和来自糖蛋白和糖肽的高甘露糖寡糖的壳二糖核心内进行水解。Endo-β-N-acetylglucosaminidase F2 (Endo F2) 水解双触角聚糖的速度大约是高甘露糖聚糖的 20 倍。Endo-β-N-acetylglucosaminidase F2 (Endo F2) 在有或没有核心岩藻糖基化的双触角结构上的活性是相同的。Endo-β-N-acetylglucosaminidase F2 (Endo F2) 对杂合或三触角和四触角寡糖没有活性。

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Endo-β-N-acetylglucosaminidase F2 Chemical Structure

Endo-β-N-acetylglucosaminidase F2 Chemical Structure

CAS No. : 37278-88-9

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Other Forms of Endo-β-N-acetylglucosaminidase F2:

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Endo-β-N-acetylglucosaminidase F2 (Endo F2), a highly specific endoglycosidase, cleaves within the chitobiose core of asparagine-linked complex biantennary and high mannose oligosaccharides from glycoproteins and glycopeptides. Endo F2 cleaves biantennary glycans at a rate approximately 20 times greater than high mannose glycans. The activity of Endo F2 is identical on biantennary structures with and without core fucosylation. Endo F2 is not active on hybrid or tri- and tetra-antennary oligosaccharides[1].

IC50 & Target

others

体外研究
(In Vitro)

使用方法
1)10 μg 的糖蛋白,加入 1 μL 的 500 mM Sodium acetate (pH=5.5),用去离子水定容到 10 μL;
2)加入 1-2 μL 的 Endo F2,轻轻吹打混匀;
3)37°C 孵育1h;
4)用 SDS-PAGE 或质谱分析;
注:孵育时间或酶量需要根据实际情况进行优化。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

CAS 号
性状

液体

颜色

Colorless to light yellow

中文名称

糖苷内切酶 F2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料

参考文献

Endo-β-N-acetylglucosaminidase F2 相关分类

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This equation is commonly abbreviated as: C1V1 = C2V2

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产品名称:
Endo-β-N-acetylglucosaminidase F2
目录号:
HY-E70133
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