Endo H, Streptomyces picatus  (Synonyms: Endo-β-N-acetylglucosaminidase H)

Endo H, Streptomyces picatus (Endo-β-N-acetylglucosaminidase H), isolated from Streptomyces plicatus, hydrolyzes the central glycosidic bond of the β1, 4-di-N-acetylchitobiose core in asparagine-linked oligosaccharides^[1].

others

使用方法
1）10 μg 的 RNase B，加入 1 μL 的 500 mM sodium acetate (pH 7.5)，用去离子水定容到 10 μL；
2）加入 1-2 μL 的 EndoH，轻轻吹打混匀；
3）37 ℃ 孵育 1 h；
4）用 SDS-PAGE 或质谱分析。
注：孵育时间或酶量需要根据实际情况进行优化。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

37278-88-9

液体

Colorless to light yellow

糖苷内切酶 H

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. V Rao, et al. Crystal structure of endo-beta-N-acetylglucosaminidase H at 1.9 A resolution: active-site geometry and substrate recognition. Structure. 1995 May 15;3(5):449-57.  [Content Brief]