Fluo-3FF AM 

Fluo-3FF AM is a low affinity (Kd = 42 μM) fluorescent Ca²⁺ sensitive indicator (Abs/Em = 462 nm/526 nm). Fluo-3FF AM is Mg²⁺ insensitive and relatively photostable. Fluo-3FF AM is an analog of Fluo-3FF AM. Fluo-3FF AM is essentially non-fluorescent, but exhibits a strong fluorescence enhancement upon entry into cells and binding to calcium.^[1]

1. Fluo-3FF AM 工作液的配制
1.1 制备储存液
用0.025% (w/v) 的 Pluronic F-127/二甲基亚砜溶液稀释 Fluo-3FF AM 去配制 1 mM 的储备液。
注：Fluo-3FF AM 储存液建议分装后于 -20℃ 或 -80℃ 避光保存。
1.2 工作液的配制
用平衡缓冲溶液，配制成 5 μM 的 Fluo-3 AM 工作液。
注：请根据实际情况调整 Fluo-3FF AM 工作液浓度，且现用现配。
2.细胞染色
2.1 将贴壁细胞培养于无菌盖玻片上。
2.2 从培养基中移出盖玻片，吸除多余培养基。
2.3 加入 100 μL 染料工作液，轻轻晃动使其完全覆盖细胞，孵育 60 分钟。
2.4 吸去染料工作液，然后在 4◦C 的 PSS 中清洗 60 分钟。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1151.80

C₅₀H₄₆Cl₂F₂N₂O₂₃

348079-13-0

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Gordienko DV, et al. Regulation of muscarinic cationic current in myocytes from guinea-pig ileum by intracellular Ca2+ release: a central role of inositol 1,4,5-trisphosphate receptors. Cell Calcium. 2004 Nov;36(5):367-86.  [Content Brief]

  [2]. Young RC, et al. Focal sarcoplasmic reticulum calcium stores and diffuse inositol 1,4,5-trisphosphate and ryanodine receptors in human myometrium. Cell Calcium. 1999 Jul-Aug;26(1-2):69-75.  [Content Brief]