Glucuronokinase (AtGlcAK)  (Synonyms: 葡萄糖醛酸激酶 (AtGlcAK))

Glucuronokinase (AtGlcAK) is a member of the GHMP-kinase. Glucuronokinase (AtGlcAK) shows unique substrate specificity for D-glucuronic acid with a K_m value of 0.7 mM. Glucuronokinase (AtGlcAK) catalyzes D-glucuronic acid and ATP to produce D-glucuronic acid-1-phosphate and ADP^[1].

产品信息
本产品为重组大肠杆菌表达，N-terminal His Tagged，分子量 40 kDa，可以用于 UDP-GlcA 合成体系，在 AtUSP 和 AtGlcAK 作用下催化 GlcA 生成 UDP-GlcA。

使用说明
1.按照反应体系加入底物，5 mM MgCl₂，可适当加入缓冲盐 (如Tris-HCl)，调节 pH 为 7.5，加入适量 Glucuronokinase (根据反应需求)；
2.混匀后，将样品置于37 ℃ 孵育 1-2 h。
注意事项
1.体系放大时候，孵育时间或酶量需要根据实际情况调整，可低速震荡混匀。
2.多数常见的生物缓冲液适用，比如 Tris 或 PBS，不在此 pH 范围的缓冲液 (如醋酸缓冲液) 可能适用，但是孵育时间或酶量需要根据实际情况进行优化。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

9026-62-4

液体

Colorless to light yellow

葡萄糖醛酸激酶 (AtGlcAK)

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Pieslinger AM, Hoepflinger MC, Tenhaken R. Cloning of Glucuronokinase from Arabidopsis thaliana, the last missing enzyme of the myo-inositol oxygenase pathway to nucleotide sugars. J Biol Chem. 2010 Jan 29;285(5):2902-10.  [Content Brief]