1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. Leucine dehydrogenase, Microorganism

Leucine dehydrogenase, Microorganism  (Synonyms: LDH, EC 1.4.1.9)

目录号: HY-P2768
COA 产品使用指南

Leucine dehydrogenase, Microorganism (EC 1.4.1.9) 能够从球形芽孢杆菌中纯化得到。Leucine dehydrogenase 催化 L-亮氨酸、L-缬氨酸、L-异亮氨酸、L-去甲缬氨酸、左旋α-氨基丁酸酯和L-去甲亮氨酸的氧化脱氨,以及它们的酮类似物的还原胺化。

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Leucine dehydrogenase, Microorganism Chemical Structure

Leucine dehydrogenase, Microorganism Chemical Structure

CAS No. : 9082-71-7

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Leucine dehydrogenase, Microorganism (EC 1.4.1.9) can be purified from Bacillus spheroides. Leucine dehydrogenase catalyzed the oxidative deamination of L-leucine, L-valine, L-isoleucine, L-norvaline, L-alpha-aminobutyrate, and L-norleucine, and the reductive amination of their keto analogues[1].

体外研究
(In Vitro)

产品信息
分子量:43 kDa (SDS-PAGE)
等电点:6.6
Km 值:2.6×10-4 M (NAD ) ,2.0×10-3 M (L-Leucine) ,6.8×10-4 M (α-Ketoisocaproate), 4.2×10-2 M (NH Cl), 2.3×10-4 M (NADH)
Ph 稳定性:6.0-11.0 (25℃, 15 hr)
最适温度:55-60℃
抑制剂:Hg2+

使用说明
可使用纯水(1 mg/mL)溶解酶,或根据具体实验参考文献 buffer 溶解。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

CAS 号
性状

固体

颜色

White to light yellow

中文名称

亮氨酸脱氢酶

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料

纯度: 95.00%

参考文献

Leucine dehydrogenase, Microorganism 相关分类

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