1. Immunology/Inflammation MAPK/ERK Pathway Stem Cell/Wnt Apoptosis
  2. Interleukin Related p38 MAPK ERK Apoptosis
  3. Lysophosphatidylcholines

Lysophosphatidylcholines  (Synonyms: 溶血磷脂酰胆碱)

目录号: HY-139414 纯度: ≥99.0%
COA 产品使用指南

Lysophosphatidylcholines 是一种具有口服活性的溶血脂质,也是氧化低密度脂蛋白 (LDL) 的一种成分。Lysophosphatidylcholines 诱导细胞损伤,产生 IL-1β 和凋亡 (apoptosis)。Lysophosphatidylcholines 对败血症有积极作用。

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Lysophosphatidylcholines Chemical Structure

Lysophosphatidylcholines Chemical Structure

CAS No. : 9008-30-4

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MCE 顾客使用本产品发表的 1 篇科研文献

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Lysophosphatidylcholines is an orally active lysolipid and a component of oxidized low density lipoprotein (LDL). Lysophosphatidylcholines induces cell injury, the production of IL-1β and apoptosis. Lysophosphatidylcholines has a proactive effect on sepsis[1][2][3][4].

IC50 & Target

IL-1β

 

ERK1

 

ERK2

 

体外研究
(In Vitro)

Lysophosphatidylcholine (3-100 μM, 24 h)降低 HUVEC 细胞活力[1]
Lysophosphatidylcholines (12.5μM, 4 h) 上调人外周血单核细胞 IL-1β 的基因表达[2]
Lysophosphatidylcholines (75μM, 24 h) 通过 p38-丝裂原活化蛋白激酶依赖机制诱导 HUVEC 凋亡[3]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay[1]

Cell Line: HUVEC
Concentration: 3 μM, 10μM, 30 μM, 100 μM
Incubation Time: 24 h
Result: Reduced HUVEC viability in a concentration-dependent manner.

Western Blot Analysis[3]

Cell Line: HUVEC
Concentration: 75 μM
Incubation Time: 24 h
Result: Showed both ERK1/2 and p38-MAPK phosphorylation.
体内研究
(In Vivo)

Lysophosphatidylcholines (0.1-20 mg/kg,口服,单次剂量) 对败血症诱导的白化 ICR 小鼠死亡具有保护作用[4]
Lysophosphatidylcholines (0.1-20 mg/kg,口服,单次剂量) 可增强细菌清除,阻断 CLP 诱导的中性粒细胞失活,提高中性粒细胞的杀菌活性[4]

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Albino ICR mice [4]
Dosage: 0.1 mg/kg, 1 mg/kg, 10 mg/kg, 20 mg/kg
Administration: Oral
Result: Provided significant protection against cecal ligation and puncture (CLP)-induced lethality at a dose of 1 mg/kg.
CAS 号
性状

固体

颜色

White to off-white

中文名称

溶血磷脂酰胆碱

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
细胞实验: 

Methanol 中的溶解度 : 25 mg/mL (超声助溶)

H2O 中的溶解度 : 10 mg/mL (超声助溶)

DMSO 中的溶解度 : < 1 mg/mL (insoluble or slightly soluble)

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息:

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

由于实验过程有损耗,建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: ≥99.0%

参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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