1. 寡核苷酸
  2. mRNA
  3. LZCap AG(3'Acm) GLuc mRNA

Gaussia荧光素酶(Gaussia Luciferase,GLuc)是体内已知最小(20KD)的可自然分泌的荧光素酶。利用Gaussia Luciferase进行荧光报告实验时,可直接利用细胞上清进行检测。该荧光素酶催化底物腔肠素的氧化反应并且发光(480nm),该反应无需ATP参与。LZCap AG(3'Acm) GLuc mRNA通过T7 转录酶和LZCap AG(3'Acm)帽类似物由线性模板一步转录合成并纯化获得。 LZCap AG(3'Acm) GLuc mRNA 适用于mRNA运送、翻译效率和转染效率等实验。

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LZCap AG(3'Acm) GLuc mRNA Chemical Structure

LZCap AG(3'Acm) GLuc mRNA Chemical Structure

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  • 生物活性

  • 纯度 & 产品资料

生物活性

Gaussia luciferase (GLuc) is the smallest known naturally secreted luciferase enzyme (20 kD) in vivo. When conducting fluorescence reporter experiments with Gaussia luciferase, detection can be directly performed using cell culture supernatant. This luciferase enzyme catalyzes the oxidation of coelenterazine to emit light (480 nm) without requiring ATP. LZCap AG(3'Acm) GLuc mRNA is synthesized and purified in a one-step transcription process using T7 RNA polymerase and LZCap AG(3'Acm) cap analog from a linear template. This mRNA is suitable for experiments related to mRNA delivery, translation efficiency, and transfection efficiency.

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料

LZCap AG(3'Acm) GLuc mRNA 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
LZCap AG(3'Acm) GLuc mRNA
目录号:
HY-164061
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