Mag-indo-1/AM 

Mag-indo-1/AM is a cell-permeable fluorescent indicator for Mg²⁺ and also for Ca²⁺ (Ex=340-390 nm, Em=410-490 nm). Mag-indo-1/AM can be used to determine intracellular Mg²⁺ and Ca²⁺ concentrations^[1][2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Measurement of intracellular Ca^2+[1]:
1. Incubate 3 mL cell suspension with 10 μM Mag-indo-1/AM for 15 min at 30℃.
2. Centrifuge the cells for 1 min at 500 rpm and resuspend in 5 mL isolation buffer (containing 1 mM CaCl₂).
3. Add 1 mM probenecid to the cell suspension to prevent dye leakage from the cytosol and store the cells (Mag-indo-1/AM-loaded cells) at room temperature until use.
4. Incubate the Mag-indo-1/AM-loaded cells at room temperature for 2 h, shake gently at 37℃ for 1.5 h, centrifuge the pellet, resuspend and store at room temperature.
6. Place a small portion of the loaded cells in an experimental chamber which is mounted on the stage of an inverted microscope.
7. Measure fluorescence of Mag-indo-1/AM (Ex=340-390 nm, Em=410 and 490 nm (the peak emissions of the Ca²⁺ bound and Ca²⁺ free forms of the indicator)). Fluorescence of the whole cell is collected on line by a photon counting system.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

730.63

C₃₃H₃₄N₂O₁₇

130926-94-2

490

340

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Griffiths EJ. Calcium handling and cell contraction in rat cardiomyocytes depleted of intracellular magnesium. Cardiovasc Res. 2000 Jul;47(1):116-23.  [Content Brief]

  [2]. Morelle B, et al. Measurement of intracellular magnesium concentration in 3T3 fibroblasts with the fluorescent indicator Mag-indo-1. Anal Biochem. 1994 Apr;218(1):170-6.  [Content Brief]