Mca-SEVNLDAEFK(Dnp) 

Mca-SEVNLDAEFK(Dnp) is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) β-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFK(Dnp) liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.

1533.50

C₆₈H₈₈N₁₄O₂₇

1802078-31-4

{Mca}-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-{Lys(Dnp)}

{Mca}-SEVNLDAEF-{Lys(Dnp)}

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Vila-Real H, et al. Peptidomimetic β-Secretase Inhibitors Comprising a Sequence of Amyloid-β Peptide for Alzheimer's Disease. J Med Chem. 2015 Jul 23;58(14):5408-18.  [Content Brief]

  [2]. Yen YC, et al. Development of an Efficient Enzyme Production and Structure-Based Discovery Platform for BACE1 Inhibitors. Biochemistry. 2019 Nov 5;58(44):4424-4435.  [Content Brief]