1. Academic Validation
  2. A reversible pH-driven DNA nanoswitch array

A reversible pH-driven DNA nanoswitch array

  • J Am Chem Soc. 2006 Feb 15;128(6):2067-71. doi: 10.1021/ja0568300.
Dongsheng Liu 1 Andreas Bruckbauer Chris Abell Shankar Balasubramanian Dae-Joon Kang David Klenerman Dejian Zhou
Affiliations

Affiliation

  • 1 Nanoscience Centre, University of Cambridge, 11 J J Thomson Avenue, Cambridge CB3 0FF, U.K. liuds@nanoctr.cn
Abstract

An array of surface-immobilized proton-fueled DNA nanomachines is reversibly actuated by cycling of the solution pH between 4.5 and 9, producing a conformational change between a four-stranded and a double-stranded structure, which elongates or shortens the separation distance between the 5' and 3' end of the DNA. By labeling the DNA 3' end with a fluorophore and immobilizing it onto a thin-gold surface through its 5' thiol modification, the nanoscale motion of the DNA produces mechanical work to lift up and bring down the fluorophore from the gold surface by at least 2.5 nm and transduces this motion into an optical "on-and-off" nanoswitch.

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