2. Academic Validation
  3. Basic cell culture conditions

Basic cell culture conditions

  • Subcell Biochem. 2006:40:345-6. doi: 10.1007/978-1-4020-4896-8_21.
Huseyin Saribasak 1,  Hiroshi Arakawa
Affiliations

Affiliation

  • 1 GSF, Institute for Molecular Radiobiology, Ingolstaedter Landstr. 1, D-85764 Neuherberg-Munich, Germany.
PMID: 17623916 DOI: 10.1007/978-1-4020-4896-8_21
Abstract

The DT40 cell line is derived from ALV (avian leukosis virus)-transformed bursal B cell of chicken. This cell line is suspension cell, and can be cultured in Cell Culture bottles and in culture plates (6-, 24- and 96-well etc.). Optimal culture temperature for the DT40 is (39 degrees C-) 41 degrees C, because chicken has higher body temperature than human and mouse. Here we summarize protocols for culture medium and condition of the DT40 cell line.

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