2. Academic Validation
  3. The phenylthiourea is a competitive inhibitor of the enzymatic oxidation of DOPA by phenoloxidase

The phenylthiourea is a competitive inhibitor of the enzymatic oxidation of DOPA by phenoloxidase

  • J Enzyme Inhib Med Chem. 2012 Feb;27(1):78-83. doi: 10.3109/14756366.2011.576010.
Alexandra D Ryazanova 1,  Alexander A Alekseev,  Irina A Slepneva
Affiliations

Affiliation

  • 1 Institute of Chemical Kinetics and Combustion, Laboratory of Chemistry and Physics of Free Radicals, Institutskaya 3, Novosibirsk, Russian Federation. razanova@kinetics.nsc.ru
PMID: 21534859 DOI: 10.3109/14756366.2011.576010
Abstract

Phenoloxidase is a key Enzyme of melanization catalyzing the oxidation of Phenols. Phenylthiourea (PTU) is the well-known and widely used inhibitor of phenoloxidase. However, the mechanism of its action is not quite clear. In the present work, the effect of PTU on the enzymatic oxidation of 3-(3,4-dihydroxyphenyl)-l-alanine (DOPA) by phenoloxidase was studied by spectrophotometric methods. The inhibition constant of PTU was estimated as 0.21 ± 0.09 µM and the competitive type of inhibition was determined for this reaction.

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