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  3. RNA SHAPE analysis in living cells

RNA SHAPE analysis in living cells

  • Nat Chem Biol. 2013 Jan;9(1):18-20. doi: 10.1038/nchembio.1131.
Robert C Spitale 1,  Pete Crisalli,  Ryan A Flynn,  Eduardo A Torre,  Eric T Kool,  Howard Y Chang
Affiliations

Affiliation

  • 1 Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California, USA.
PMID: 23178934 DOI: 10.1038/nchembio.1131
Abstract

RNA structure has important roles in practically every facet of gene regulation, but the paucity of in vivo structural probes limits current understanding. Here we design, synthesize and demonstrate two new chemical probes that enable selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) in living cells. RNA structures in human, mouse, fly, yeast and Bacterial cells are read out at single-nucleotide resolution, revealing tertiary contacts and RNA-protein interactions.

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