2. Academic Validation
  3. Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni

Isolation, NMR Spectral Analysis and Hydrolysis Studies of a Hepta Pyranosyl Diterpene Glycoside from Stevia rebaudiana Bertoni

  • Biomolecules. 2013 Sep 30;3(4):733-40. doi: 10.3390/biom3040733.
Venkata Sai Prakash Chaturvedula 1,  Steven Chen 2,  Oliver Yu 3,  Guohong Mao 4
Affiliations

Affiliations

  • 1 Natural Ingredient Development, Blue California, 30111 Tomas, Rancho Santa Margarita, CA 92688, USA. saipc@bluecal-ingredients.com.
  • 2 Natural Ingredient Development, Blue California, 30111 Tomas, Rancho Santa Margarita, CA 92688, USA. steven@bluecal-ingredients.com.
  • 3 Natural Ingredient Development, Blue California, 30111 Tomas, Rancho Santa Margarita, CA 92688, USA. oliver@bluecal-ingredients.com.
  • 4 Natural Ingredient Development, Blue California, 30111 Tomas, Rancho Santa Margarita, CA 92688, USA. guohongmao@yahoo.com.
PMID: 24970189 DOI: 10.3390/biom3040733
Abstract

From the commercial extract of the leaves of Stevia rebaudiana Bertoni, a minor steviol glycoside, 13-[(2-O-β-D-glucopyranosyl-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl)oxy] ent-kaur-16-en-19-oic acid-[(2-O-(3-O-β-D-glucopyranosyl-α-L-rhamnopyranosyl)-3-O-β-D-glucopyranosyl-β-D-glucopyranosyl) ester] (1); also known as rebaudioside O having seven sugar units has been isolated. Its structural characterization has been achieved by the extensive 1D (1H and 13C), and 2D NMR (COSY, HMQC, HMBC) as well as mass spectral data. Further, hydrolysis studies were performed on rebaudioside O using acid and enzymatic methods to identify aglycone and sugar residues in its structure as well as their configurations.

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