2. Academic Validation
  3. Bee Venom Acupuncture Alleviates Experimental Autoimmune Encephalomyelitis by Upregulating Regulatory T Cells and Suppressing Th1 and Th17 Responses

Bee Venom Acupuncture Alleviates Experimental Autoimmune Encephalomyelitis by Upregulating Regulatory T Cells and Suppressing Th1 and Th17 Responses

  • Mol Neurobiol. 2016 Apr;53(3):1419-1445. doi: 10.1007/s12035-014-9012-2.
Min Jung Lee 1 2,  Minhee Jang 1 2,  Jonghee Choi 2 3,  Gihyun Lee 4,  Hyun Jung Min 5 6 7,  Won-Seok Chung 8,  Jong-In Kim 9,  Youngheun Jee 10 11,  Younbyoung Chae 12,  Sung-Hoon Kim 1,  Sung Joong Lee 5 6 7,  Ik-Hyun Cho 13 14 15
Affiliations

Affiliations

  • 1 Department of Cancer Preventive Material Development, College of Oriental Medicine, Kyung Hee University, Seoul, 130-701, Republic of Korea.
  • 2 Department of Convergence Medical Science, College of Oriental Medicine, Kyung Hee University, Seoul, 130-701, Republic of Korea.
  • 3 Brain Korea 21 Plus Program, Kyung Hee University, Seoul, 130-701, Republic of Korea.
  • 4 Department of Physiology, College of Oriental Medicine, Kyung Hee University, Seoul, 130-701, Republic of Korea.
  • 5 Department of Neuroscience and Physiology, School of Dentistry, Seoul National University, Seoul, 110-799, Republic of Korea.
  • 6 Dental Research Institute, School of Dentistry, Seoul National University, Seoul, 110-799, Republic of Korea.
  • 7 Brain Korea 21 Plus Program, School of Dentistry, Seoul National University, Seoul, 110-799, Republic of Korea.
  • 8 Department of Rehabilitation Medicine, College of Oriental Medicine, Kyung Hee University, Seoul, 130-701, Republic of Korea.
  • 9 Department of Acupuncture and Moxibustion, College of Oriental Medicine, Kyung Hee University, Seoul, 130-701, Republic of Korea.
  • 10 College of Veterinary Medicine, Jeju National University, Jeju, 690-756, Republic of Korea.
  • 11 Applied Radiological Science Institute, Jeju National University, Jeju, 690-756, Republic of Korea.
  • 12 Acupuncture and Meridian Science Research Center, College of Oriental Medicine, Kyung Hee University, Seoul, 130-701, Republic of Korea.
  • 13 Department of Convergence Medical Science, College of Oriental Medicine, Kyung Hee University, Seoul, 130-701, Republic of Korea. ihcho@khu.ac.kr.
  • 14 Brain Korea 21 Plus Program, Kyung Hee University, Seoul, 130-701, Republic of Korea. ihcho@khu.ac.kr.
  • 15 Institute of Oriental Medicine, College of Oriental Medicine, Kyung Hee University, Seoul, 130-701, Republic of Korea. ihcho@khu.ac.kr.
PMID: 25579380 DOI: 10.1007/s12035-014-9012-2
Abstract

The protective and therapeutic mechanism of bee venom acupuncture (BVA) in neurodegenerative disorders is not clear. We investigated whether treatment with BVA (0.25 and 0.8 mg/kg) at the Zusanli (ST36) acupoints, located lateral from the anterior border of the tibia, has a beneficial effect in a myelin basic protein (MBP)(68-82)-induced acute experimental autoimmune encephalomyelitis (EAE) rat model. Pretreatment (every 3 days from 1 h before immunization) with BVA was more effective than posttreatment (daily after immunization) with BVA with respect to clinical signs (neurological impairment and loss of body weight) of acute EAE rats. Treatment with BVA at the ST36 acupoint in normal rats did not induce the clinical signs. Pretreatment with BVA suppressed demyelination, glial activation, expression of cytokines [interferon (IFN)-γ, IL-17, IL-17A, tumor necrosis factor-alpha (TNF-α), and IL-1β], chemokines [RANTES, monocyte chemotactic protein-1 (MCP-1), and macrophage inflammatory protein (MIP)-1α], and inducible nitric oxide synthase (iNOS), and activation of p38 mitogen-activated protein kinase (MAPK) and nuclear factor (NF)-κB (p65 and phospho-IκBα) signaling pathways in the spinal cord of acute EAE rats. Pretreatment with BVA decreased the number of CD4(+), CD4(+)/IFN-γ(+), and CD4(+)/IL-17(+) T cells, but increased the number of CD4(+)/Foxp3(+) T cells in the spinal cord and lymph nodes of acute EAE rats. Treatment with BVA at six placebo acupoints (SP9, GB39, and four non-acupoints) did not have a positive effect in acute EAE rats. Interestingly, onset and posttreatment with BVA at the ST36 acupoint markedly attenuated neurological impairment in myelin oligodendrocyte glycoprotein (MOG)(35-55)-induced chronic EAE mice compared to treatment with BVA at six placebo acupoints. Our findings strongly suggest that treatment with BVA with ST36 acupoint could delay or attenuate the development and progression of EAE by upregulating regulatory T cells and suppressing T-helper (Th) 17 and Th1 responses. These results warrant further investigation of BVA as a treatment for autoimmune disorders of the central nervous system.

Keywords

Bee venom acupuncture; Experimental autoimmune encephalomyelitis; Immune cell.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z