2. Academic Validation
  3. Lupalbigenin from Derris scandens Sensitizes Detachment-induced Cell Death in Human Lung Cancer Cells

Lupalbigenin from Derris scandens Sensitizes Detachment-induced Cell Death in Human Lung Cancer Cells

  • Anticancer Res. 2015 May;35(5):2827-34.
Apisara Ausawasamrit 1,  Nattawut Itthiwarapornkul 1,  Chatchai Chaotham 2,  Suchada Sukrong 3,  Pithi Chanvorachote 4
Affiliations

Affiliations

  • 1 Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
  • 2 Department of Biochemistry and Microbiology, Chulalongkorn University, Bangkok, Thailand Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.
  • 3 Department of Pharmacognosy and Pharmaceutical Botany, Chulalongkorn University, Bangkok, Thailand Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand pithi_chan@yahoo.com Suchada.su@chula.ac.th.
  • 4 Department of Pharmacology and Physiology, Chulalongkorn University, Bangkok, Thailand Cell-Based Drug and Health Product Development Research Unit, Chulalongkorn University, Bangkok, Thailand Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand pithi_chan@yahoo.com Suchada.su@chula.ac.th.
PMID: 25964563
Abstract

Background/aim: The ability of Cancer cells to resist to anoikis has been shown to augment Cancer cell metastasis in many cancers. In search for potential substances for anti-metastatic approaches, this study aimed to investigate anoikis-sensitizing activity of lupalbigenin, extracted from Derris scandens.

Materials and methods: Human lung Cancer cells were treated with non-cytotoxic concentrations of lupalbigenin in a detachment condition. Anoikis was evaluated at various time points using MTT viability assays. The effect of lupalbigenin on anchorage-independent growth was performed by soft-agar assay. The survival signaling proteins, as well as regulatory proteins of Apoptosis and metastasis, were examined by western blot analysis.

Results: Lupalbigenin treatment significantly down-regulated survival proteins, including protein kinase B (pAKT/Akt) and extracellular signal-regulated kinase (PERK/ERK), as well as anti-apoptotic protein B-cell lymphoma 2 (Bcl-2), resulting in the enhancement of the cellular response to anoikis and the decrease of growth and survival in an anchorage-independent condition.

Conclusion: Lupalbigenin sensitizes detachment-induced cell death in human lung Cancer cell through down-regulation of pro-survival proteins.

Keywords

Derris scandens; Lung cancer; anoikis; lupalbigenin; pro-survival pathway.

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