2. Academic Validation
  3. Palmitoleic acid induces the cardiac mitochondrial membrane permeability transition despite the presence of L-carnitine

Palmitoleic acid induces the cardiac mitochondrial membrane permeability transition despite the presence of L-carnitine

  • Biochem Biophys Res Commun. 2015 Jul;463(1-2):29-36. doi: 10.1016/j.bbrc.2015.05.011.
Eri Oyanagi 1,  Masataka Uchida 2,  Takeshi Miyakawa 2,  Motohiko Miyachi 3,  Hidetaka Yamaguchi 4,  Kuniatsu Nagami 4,  Kozo Utsumi 5,  Hiromi Yano 6
Affiliations

Affiliations

  • 1 Department of Health Promotion and Exercise, National Institute of Health and Nutrition, Tokyo, Japan; Research Institute of Health and Welfare, Kibi International University, Takahashi, Japan.
  • 2 Department of Health & Sports Science, Kawasaki University of Medical Welfare, Kurashiki, Japan.
  • 3 Department of Health Promotion and Exercise, National Institute of Health and Nutrition, Tokyo, Japan.
  • 4 Research Institute of Health and Welfare, Kibi International University, Takahashi, Japan.
  • 5 Department of Cytology & Histology, Okayama University Graduate School, Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
  • 6 Department of Health & Sports Science, Kawasaki University of Medical Welfare, Kurashiki, Japan. Electronic address: yanohiro@mw.kawasaki-m.ac.jp.
PMID: 25983324 DOI: 10.1016/j.bbrc.2015.05.011
Abstract

Although palmitoleic acid (C16:1) is associated with arrhythmias, and increases in an age-dependent matter, the effects of L-carnitine, which is essential for the transport of long-chain fatty acids into the mitochondria, are unclear. It has been postulated that L-carnitine may attenuate palmitate (C16:0)-induced mitochondrial dysfunction and the Apoptosis of cardiomyocytes. The aim of this study was to elucidate the activity of L-carnitine in the prevention of the palmitoleic acid-induced mitochondrial membrane permeability transition and cytochrome c release using isolated cardiac mitochondria from rats. Palmitoleoyl-CoA-induced mitochondrial respiration was not accelerated by L-carnitine treatment, and this respiration was slightly inhibited by oligomycin, which is an inhibitor of ATP Synthase. Despite pretreatment with L-carnitine, the mitochondrial membrane potential decreased and mitochondrial swelling was induced by palmitoleoyl-CoA. In the presence of a combination of L-carnitine and tiron, a free radical scavenger, there was attenuated mitochondrial swelling and cytochrome c release following palmitoleoyl-CoA treatment. We concluded that palmitoleic acid, but not palmitate, induces the cardiac mitochondrial membrane permeability transition despite the presence of L-carnitine.

Keywords

Cytochrome c; Membrane potential; Palmitoleoyl-CoA; Palmitoyl-CoA; Swelling; Tiron.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z