2. Academic Validation
  3. ATP-association to intrabacterial nanotransportation system in Vibrio cholerae

ATP-association to intrabacterial nanotransportation system in Vibrio cholerae

  • Med Mol Morphol. 2015 Dec;48(4):225-34. doi: 10.1007/s00795-015-0105-4.
Yuji Matsuzaki 1,  Hong Wu 1,  Takashi Nakano 1,  Takashi Nakahari 2 3,  Kouichi Sano 4
Affiliations

Affiliations

  • 1 Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka, 569-8686, Japan.
  • 2 Department of Physiology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka, 569-8686, Japan.
  • 3 Department of Molecular Cell Physiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto, 602-8566, Japan.
  • 4 Department of Microbiology and Infection Control, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka, 569-8686, Japan. ksano@art.osaka-med.ac.jp.
PMID: 25986680 DOI: 10.1007/s00795-015-0105-4
Abstract

Vibrio cholerae colonizes the lumen of the proximal small intestine, which has an alkaline environment, and secretes cholera toxin (CT) through a type II secretion machinery. V. cholerae possesses the intrabacterial nanotransportation system (ibNoTS) for transporting CT from the inner portion toward the peripheral portion of the cytoplasm, and this system is controlled by extrabacterial pH. Association of ATP with ibNoTS has not yet been examined in detail. In this study, we demonstrated by immunoelectron microscopy that ibNoTS of V. cholerae under the extrabacterial alkaline condition was inhibited by ATP inhibitors, 2,4-dinitrophenol (DNP), a protonophore, or 8-amino-adenosine which produces inactive form of ATP. The inhibition of CT transport can be reversed by neutralization of DNP. Those inhibitions were associated with decrease of CT secretion by which ibNoTS followed. We propose that ATP closely associates with V. cholerae ibNoTS for transporting CT.

Keywords

ATP; Cholera toxin; Immunoelectron microscopy; Nanotransportation; Vibrio cholerae.

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