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  3. Genome editing of CXCR4 by CRISPR/cas9 confers cells resistant to HIV-1 infection

Genome editing of CXCR4 by CRISPR/cas9 confers cells resistant to HIV-1 infection

  • Sci Rep. 2015 Oct 20;5:15577. doi: 10.1038/srep15577.
Panpan Hou 1 2,  Shuliang Chen 1,  Shilei Wang 2,  Xiao Yu 2,  Yu Chen 2,  Meng Jiang 3,  Ke Zhuang 4,  Wenzhe Ho 4,  Wei Hou 1,  Jian Huang 5,  Deyin Guo 1
Affiliations

Affiliations

  • 1 School of Basic Medical Sciences, Wuhan University, Wuhan, 430071, PR China.
  • 2 College of Life Sciences, Wuhan University, Wuhan, 430072, PR China.
  • 3 Renmin Hospital of Wuhan University, Wuhan 430060, PR China.
  • 4 The Center for Animal Experiment and ABSL-3 Laboratory, Wuhan University School of Medicine, Wuhan, 430071, PR China.
  • 5 Department of Pathology and Laboratory Medicine, School of Medicine, Temple University, Philadelphia, PA 19103.
PMID: 26481100 DOI: 10.1038/srep15577
Abstract

Genome editing via CRISPR/Cas9 has become an efficient and reliable way to make precise, targeted changes to the genome of living cells. CXCR4 is a co-receptor for the human immunodeficiency virus type 1 (HIV-1) Infection and has been considered as an important therapeutic target for AIDS. CXCR4 mediates viral entry into human CD4(+) cells by binding to envelope protein, gp120. Here, we show that human CXCR4 gene is efficiently disrupted by CRISPR/Cas9-mediated genome editing, leading to HIV-1 resistance of human primary CD4(+) T cells. We also show that the Cas9-mediated ablation of CXCR4 demonstrated high specificity and negligible off-target effects without affecting cell division and propagation. The precise and efficient genome editing of CXCR4 will provide a new strategy for therapeutic application against HIV-1 Infection.

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