2. Academic Validation
  3. Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth

Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth

  • J Microbiol Methods. 2016 Jun:125:40-2. doi: 10.1016/j.mimet.2016.03.018.
Kássia de Carvalho Dias 1,  Paula Aboud Barbugli 2,  Carlos Eduardo Vergani 3
Affiliations

Affiliations

  • 1 Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program-Araraquara School of Dentistry, UNESP-Univ. Estadual Paulista, Centro, 14801903 Araraquara, SP, Brazil. Electronic address: kassiaodonto@hotmail.com.
  • 2 Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program-Araraquara School of Dentistry, UNESP-Univ. Estadual Paulista, Centro, 14801903 Araraquara, SP, Brazil. Electronic address: pabfarma@yahoo.com.br.
  • 3 Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program-Araraquara School of Dentistry, UNESP-Univ. Estadual Paulista, Centro, 14801903 Araraquara, SP, Brazil. Electronic address: vergani@foar.unesp.br.
PMID: 27060444 DOI: 10.1016/j.mimet.2016.03.018
Abstract

This study assessed the effect of the buffers 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 3-(N-morpholino) propanesulfonic acid (MOPS) on keratinocyte cell viability and microbial growth. It was observed that RPMI buffered with HEPES, supplemented with l-glutamine and sodium bicarbonate, can be used as a more suitable medium to promote co-culture.

Keywords

Candida albicans; Cell survival; HEPES; Keratinocytes; Staphylococcus aureus.

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