2. Academic Validation
  3. Trifloxystrobin induces tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis in HaCaT, human keratinocyte cells

Trifloxystrobin induces tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis in HaCaT, human keratinocyte cells

  • Drug Chem Toxicol. 2017 Jan;40(1):67-73. doi: 10.1080/01480545.2016.1174871.
Yoonjeong Jang 1,  Ah Young Lee 1,  Seung-Hee Chang 1,  Sang-Hee Jeong 2,  Kyung-Hun Park 3,  Min-Kyoung Paik 3,  Nam-Joon Cho 3,  Ji-Eun Kim 1,  Myung-Haing Cho 1 4 5 6 7
Affiliations

Affiliations

  • 1 a Laboratory of Toxicology , BK21 PLUS Program for Creative Veterinary Science Research, Research Institute for Veterinary Science and College of Veterinary Medicine, Seoul National University , Seoul , Korea.
  • 2 b Department of BioApplied Toxicology , Hoseo Toxicology Research Center, Hoseo University , Asan , Korea.
  • 3 c Rural Development Administration, National Institute of Agricultural Science , Jeonju , Korea.
  • 4 d Graduate School of Convergence Science and Technology, Seoul National University , Suwon , Korea.
  • 5 e Graduate Group of Tumor Biology, Seoul National University , Seoul , Korea.
  • 6 f Advanced Institute of Convergence Technology, Seoul National University , Suwon , Korea , and.
  • 7 g Institute of GreenBio Science Technology, Seoul National University , Pyeongchang , Korea.
PMID: 27149887 DOI: 10.1080/01480545.2016.1174871
Abstract

As the outermost layer of the body, the skin plays an important role in exposure to pesticides, which could have negative impacts on human health. Trifloxystrobin is a widely used fungicide of the strobilurin class, however, there is little information regarding the skin contact-associated toxic mechanism. Therefore, the present study was performed in order to identify the skin toxicity mechanism of trifloxystrobin using HaCaT (keratinocyte of human skin) cells. Following 24 or 48 h treatment, cell viability, and subsequent Annexin V-FITC/propidium iodide assay, TUNEL assay and Western blotting were performed to investigate the cell death mechanism of trifloxystrobin. Exposure to trifloxystrobin resulted in diminished viability of HaCaT cells in both a time- and concentration-dependent manner. The cell death was derived through apoptotic pathways in the HaCaT cells. Furthermore, we explored the effect of trifloxystrobin on TRAIL-mediated extrinsic Apoptosis using siRNA transfection. Knockdown of Death Receptor 5 suppressed trifloxystrobin-provoked Apoptosis. These results indicate that trifloxystrobin induces TRAIL-mediated Apoptosis and has an inhibitory effect in keratinocytes that can interfere with the barrier function and integrity of the skin. This could be proposed as a mechanism of skin toxicity by trifloxystrobin and considered in the management of pesticide exposure.

Keywords

TRAIL-mediated apoptosis; Trifloxystrobin; keratinocyte.

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