1. Academic Validation
  2. Long noncoding RNA EGOT negatively affects the antiviral response and favors HCV replication

Long noncoding RNA EGOT negatively affects the antiviral response and favors HCV replication

  • EMBO Rep. 2016 Jul;17(7):1013-28. doi: 10.15252/embr.201541763.
Elena Carnero 1 Marina Barriocanal 1 Celia Prior 1 Juan Pablo Unfried 1 Victor Segura 2 Elizabeth Guruceaga 2 Mónica Enguita 1 Cristian Smerdou 1 Pablo Gastaminza 3 Puri Fortes 4
Affiliations

Affiliations

  • 1 Department of Gene Therapy and Hepatology, Center for Applied Medical Research (CIMA) and IdiSNA Navarra Institute for Health Research University of Navarra, Pamplona, Spain.
  • 2 Bioinformatics Unit, CIMA and IdisNA University of Navarra, Pamplona, Spain.
  • 3 National Center of Biotechnology CNB/CSIC, Madrid, Spain.
  • 4 Department of Gene Therapy and Hepatology, Center for Applied Medical Research (CIMA) and IdiSNA Navarra Institute for Health Research University of Navarra, Pamplona, Spain pfortes@unav.es.
Abstract

The role of long noncoding RNAs (lncRNAs) in viral Infection is poorly studied. We have identified hepatitis C virus (HCV)-Stimulated lncRNAs (CSRs) by transcriptome analysis. Interestingly, two of these CSRs (PVT1 and UCA1) play relevant roles in tumorigenesis, providing a novel link between HCV Infection and development of liver tumors. Expression of some CSRs seems induced directly by HCV, while Others are upregulated by the Antiviral response against the virus. In fact, activation of pathogen sensors induces the expression of CSR32/EGOT RIG-I and the RNA-activated kinase PKR sense HCV RNA, activate NF-κB and upregulate EGOT EGOT is increased in the liver of patients infected with HCV and after Infection with influenza or Semliki Forest virus (SFV). Genome-wide guilt-by-association studies predict that EGOT may function as a negative regulator of the Antiviral pathway. Accordingly, EGOT depletion increases the expression of several interferon-stimulated genes and leads to decreased replication of HCV and SFV Our results suggest that EGOT is a lncRNA induced after Infection that increases viral replication by antagonizing the Antiviral response.

Keywords

EGOT; HCV; PKR; SFV; lncRNAs.

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